Nanocurcumin Innovation as an Anti-Apoptosis of Ovarian Granulosa Cells in White Rats Exposed to Lead Acetate (PbAc)
Article Sidebar
Main Article Content
Abstract
Introduction: Exposure to Pb causes increased apoptosis of ovarian granulosa cells through oxidative stress mechanism. Curcumin has protective effects on reproductive organs, anti-apoptotic, antioxidant in normal cells. Curcumin in innovated nano form can function as an effective anti-apoptosis in ovarian granulosa cells of rats due to PbAc exposure.
Methods: Thirty female rats were divided into 3 groups, the negative control group (the rats receiving distilled water, in each 90 minutes receiving corn oil), positive control group (the rats receiving PbAc of 30 mg/kg BW, in each 90 minutes receiving corn oil), the experimental group, in which the rats receiving PbAc of 30 mg/kg BW, and in each 90 minutes receiving nanocurcumin of 200 mg/kg BW. All groups received treatment orally once a day for 30 days. On day 31 the rats to granulosa cell apoptosis examination using Tunnel method.
Results: Rate of apoptosis was in the positive control group (5.4 ± 0.8%/micro) and the lowest was in the experimental group (1.1 ± 0.5%/micro) and the negative control group (1.2 ± 0.6). The experimental group showed the same p value as the negative control group (p = .095) and different p value (p = .010) from the positive control group. These findings indicated that the innovation of curcumin in nano form at a dose of 200 mg/KgBW reduced apoptosis of rat ovarian granulosa cells due to PbAc exposure.
Conclusion: The innovation of curcumin in nano form has the potential as an effective natural anti-apoptosis in rats ovarian granulosa cells exposed to PbAc.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Babali Nursing Research (BNR) agree to the following terms:
-
Authors retain copyright and grant BNR the right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to remix, adapt and build upon the work with an acknowledgment of the work's authorship and of the initial publication in BNR.
-
Authors are permitted to copy and redistribute the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in BNR.
References
[2] R. Mohebbati, A. Anaeigoudari, and M. R. Khazdair, “The effects of Curcuma longa and curcumin on reproductive systems,” Endocr. Regul., vol. 51, no. 4, pp. 220–228, 2017, doi: 10.1515/enr-2017-0024.
[3] R. Banjerdpongchai, M. Suttajit, and P. Kongtawelert, “Effect of curcumin on HL60 cell apoptosis induced by doxorubicin,” Chiang Mai Med Bull, vol. 41, no. 2, pp. 59–66, 2002.
[4] S. Shishodia, M. M. Chaturvedi, and B. B. Aggarwal, “Role of curcumin in cancer therapy,” Curr Probl Cancer, vol. 31, no. 4, pp. 243–305, 2007.
[5] D. M. . Bodhankar and S. Chikhle, “Various Approaches Towards Enhancement of Bioavailability of Curcumin - A Potent Phytochemical,” World J. Pharm. Res., vol. 8, no. 1, pp. 606–626, 2018, doi: 10.20959/wjpr20191-13932.
[6] M. . Pan, T. . Huang, and J. . Lin, “Biotransformation of curcumin through reduction and glucuronidation in mice,” Drug Metab. Dispos., vol. 27, no. 1, pp. 486–494, 1999.
[7] P. Rajsekhar, R. . Bharani, K. . Angel, and M. Ramachandran, “Curcumin Nanoparticles?: A Therapeutic Review,” Res. J. Pharm. Biol. Chem. Sci., vol. 6, no. 5, pp. 1180–1186, 2015.
[8] S. Prasad, A. K. Tyagi, and B. B. Aggarwal, “Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: The golden pigment from golden spice,” Cancer Res. Treat., vol. 46, no. 1, pp. 2–18, 2014.
[9] M. Shishu, Maheswari, “Comparative Bioavaibility of curcumin, turmeric, and biocurcumax, in tradisional vehicles using non-everted rat intestinal sac model,” J. Funct. Food, vol. 2, pp. 60–65, 2010.
[10] W. Nuraeni, I. Daruwati, E. Maria W., and E. Sriyani, M., “Verification of the performance of the Horiba LB-550 particle Sinze Analyzer (PSA) for determining the size distribution of nanoparticles,” Proc. Natl. Semin. Nucl. Sci. Technol., 2013.
[11] L. Chabib, R. Martien, and H. Ismail, “Formulation of nanocurcumin using low viscosity chitosan polymer and its cellular uptake study into T47D cells,” Indones. J. Pharm., vol. 23, no. 1, pp. 27–35, 2013.
[12] D. de M. Carvalho, K. P. Takeuchi, de M. Geraldine, R. M., C. J., and M. C. L. Torres, “Production, solubility and antioxidant activity of curcumin nanosuspension,” Food Sci. Technol., vol. 35, no. 1, pp. 115–119, 2015, doi: 10.1590/1678-457X.6515.
[13] A. Albanese, P. S. Tang, and W. C. W. Chan, “The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems. Annual Review of Biomedical Engineering,” Annu. Rev. Biomed. Eng., vol. 14, no. 1, pp. 1–16, 2012, doi: 10.1146/annurev-bioeng-071811-150124.
[14] AL-Megrin, W. A., D. Soliman, R. B. Kassab, D. M. Metwally, D. M. Dina, and M. F. El-Khadragy, “Coenzyme Q10 Activates the Antioxidant Machinery and Inhibits the Inflammatory and Apoptotic Cascades Against Lead Acetate-Induced Renal Injury in Rats,” Front. Physiol., vol. 11, no. 2, pp. 1–13, 2020, doi: 10.3389/fphys.2020.00064.
[15] M. A. O’Brien and R. Kirby, “Apoptosis: A review of pro-apoptotic and anti- apoptotic pathways and dysregulation in disease,” J. Vet. Emerg. Crit. Care, vol. 18, no. 6, pp. 572–585, 2008, doi: 10.1111/j.1476-4431.2008.00363.x.
[16] S. . Sudjarwo, C. Anwar, G. Wardani, K. Eraiko, and Koerniasari, “Antioxidant and anti-caspase 3 effect of chitosan-Pinus merkusii extract nanoparticle against lead acetate-induced testicular toxicity in rat,” Asian Pacific J. Reprod., vol. 8, no. 1, pp. 13–19, 2019, doi: https://doi.org/10.4103/2305-0500.250418.
[17] Kabeer, A., M. Muhammad Mailafiya, A. Danmaigoro, E. Abdul Rahim, B. Bakar, and M. Z. A, “Therapeutic potential of curcumin against lead-induced toxicity: A review,” Biomed. Res. Ther., vol. 6, no. 3, pp. 3053–3066, 2019, doi: https://doi.org/10.15419/bmrat.v6i3.528.
[18] N. Singh, A. Kumar, V. K. Gupta, and B. Sharma, “Biochemical and Molecular Bases of Lead-Induced Toxicity in Mammalian Systems and Possible Mitigations,” Chem. Res. Toxicol., vol. 31, no. 10, pp. 1009–1021, 2018, doi: https://doi.org/10.1021/acs.chemrestox.8b00193.
[19] M. A. Assi, M. Noor, M. Hezmee, A. W. Haron, M. Yusof, and M. Sabri, “The detrimental effects of lead on human and animal health,” Vet. World, vol. 9, pp. 660–671, 2016, doi: https://doi.org/10.14202/vetworld.2016.660-671.
[20] Dumitrescu, E., V. Chiurciu, F. Muselin, R. Popescu, D. Brezovan, and R. T. Cristina, “Effects of long-term exposure of female rats to low levels of lead: Ovary and uterus histological architecture changes,” Turkish J. Biol., vol. 39, no. 2, pp. 284–289, 2015, doi: https://doi.org/10.3906/biy-1407-6.
[21] M. Majeed, V. Badmaev, U. Shivakumar, and R. Rajendranm, Curcuminoids - Antioxidant Phytonutrients. NutriScience Pub, 1995.
[22] P. Suryohudo, Molecular Medicine. Jakarta: Sagung Seto, 2000.
[23] M. Redza-Dutordoir and D. A. Averill-Bates, “Activation of apoptosis signalling pathways by reactive oxygen species,” Biochim. Biophys. Acta-Molecular Cell Res., vol. 1863, no. 12, pp. 2977–2992, 2016, doi: 10.1016/j.bbamcr.2016.09.012.